Research Associate II, Upstream CMC

The Organization

What's happening at Entrada Therapeutics? Entrada is a clinical-stage biopharmaceutical company aiming to transform the lives of patients by establishing a new class of medicines that engage intracellular targets that have long been considered inaccessible.

Our Endosomal Escape Vehicle (EEV™)-therapeutics are designed to enable the efficient intracellular delivery of a wide range of therapeutics into a variety of organs and tissues, resulting in an improved therapeutic index. Through this proprietary, versatile and modular approach, Entrada is advancing a robust development portfolio of RNA-, antibody- and enzyme-based programs for the potential treatment of neuromuscular, ocular, metabolic and immunological diseases, among others.

Our lead oligonucleotide programs are in development for the potential treatment of people living with Duchenne who are exon 44, 45 and 50 skipping amenable. We have also partnered to develop a clinical-stage program, VX-670, for myotonic dystrophy type 1.

We are a tight-knit team of experts and leaders in both therapeutic development and rare diseases and are excited to grow and attract colleagues who are ready to join a high-energy, dedicated team that likes to get things done and dramatically improve the lives of patients and their families.

The Perfect Addition to Our Team

You are a versatile, self-motivated, and eager Research Associate I who is looking forward to rolling up your sleeves and contributing to all aspects of the CMC upstream team. You thrive in a fast-paced environment and able to juggle competing priorities. You work successfully in a team environment with strong interpersonal skills to effectively build relationships with cross functional groups. 

The Opportunity

The Research Associate I will support high-throughput screening, microbial/yeast fermentation, and primary recovery. They will be responsible for the high-throughput screens to support construct screening and fermentation/cell culture, as well as purification to generate materials for in vitro and in vivo studies. The ideal candidate will work side-by-side with biologics discovery, downstream, analytical, and pharmacology team members to identify the lead candidate and nominate candidate selections.

Responsibilities

• Set-up, inoculate, and operate meticulously shake flasks, small- and medium-scale mammalian cell culture, yeast and bacterial fermentations.
• Run deep well plates, shake flask for construct screening and process optimization.
• Perform primary recovery operations such as homogenization, centrifugation, inclusion body wash, solubilization, depth filtration, and refolding.
• Run SDS-PAGE gels, western blots, and protein concentration assay.
• Operate AKTA Avant, Bio-Rad NGC FPLC systems for protein purification (as needed)
• Maintain upstream lab equipment, lab space, inventory of upstream raw materials and consumables.
• Record experimental details and observations in the laboratory notebooks in a timely manner.
• Present experimental design, data, and research findings in internal team meetings.

The Necessities

At Entrada, our passion for science, our devotion to patients and our values drives our behavior:

• Humanity - We genuinely care about patients and about one another.
• Tenacity - We are relentless and persistent in the pursuit of developing therapies for patients.
• Creativity - We are creative problem solvers.
• Collaboration - We are more than the sum of our parts.
• Curiosity - We have a growth mindset and push conventional thought and theory.

To thrive on our team, you will need to come with:

• BS or MS degree in biochemical engineering, biotechnology, biochemistry, or a related field with at least 2+ years of wet lab experience in an academic or industry environment.
• Experience working with E. coli or/and Pichia pastoris.
• Experience running SDS-PAGE and western blots for monitoring protein production.
• Experience with primary recovery operations such as centrifugation, depth filtration, homogenization, solubilization, refolding.
• Experience with TFF operation to concentrate and diafilter recombinant proteins (a plus).
• Strong theoretical understanding of the principles of fermentation/cell culture process.
• Strong communication skill (oral and written) and ability to work effectively across functions.
• Ability to proactively prioritize and help drive experimental designs forward.
• Growth mindset with ability to adapt to changing priorities.
• Able to work during outside of regular business hours and weekends. #LI-DS1

The Perks

By becoming a team member here at Entrada, you’ll have access to competitive health, dental, and vision coverage, as well as life insurance, and short term and long-term disability insurance. We value work life balance, you'll benefit from discretionary time off, paternity leave, and an excellent 401(k) package. We also offer a generous transportation stipend to commute as you wish. Our location in the vibrant and growing Seaport District is close to all the best that the city of Boston has to offer.

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

Third Party Staffing Agencies

Entrada does not accept unsolicited resumes from any source other than directly from candidates. For the protection of all parties involved in the recruiting process, resumes will only be accepted from recruiters/agencies if a signed agreement is in place at the inception of the recruiting effort and authorized for a specified position. Unsolicited resumes sent to Entrada from recruiters/agencies do not constitute any type of relationship between the recruiter/agency and Entrada and do not obligate Entrada to pay fees if we hire from those resumes.

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